Thawing Instructions for Canine Cryobank, Inc. Frozen Semen


NOTE: Not all frozen semen is processed, packaged, or thawed the same way!
Please READ AND FOLLOW THESE INSTRUCTIONS CAREFULLY! Call us with any questions.

PREPARATION
A.
Prepare 37-38 degree Centigrade water baths. Two large styrofoam cups with four inches of water work well.
B.
Warm insemination rod, syringe, and needle to 37-38 degrees Centigrade in an incubator. DO NOT SUBMERGE IN WATER BATH!
THAW PROCEDURES
1.
Open shipping tank. Locate aluminum cane labeled "Thaw media". Remove small vial containing thaw media and warm to room temperature. Estimated time: 10-15 minutes.
2.
Put 1/2 cc. of room temperature THAW MEDIA in each culture tube. (One culture tube with 1/2 cc thaw media for each straw.) Place tubes in 37-38 degree centigrade water bath. Keep these tubes at 37-38 degrees centigrade THIS IS IMPORTANT!
3.
Have 37-38 degree centigrade water bath ready for thawing semen.
WARNING! REMOVE STRAW ONLY WHEN YOU ARE READY TO THAW!
Each semen straw must be thawed at 37-38 degrees C. Monitor temperature carefully for each straw.
4.
Use tweezers or hemostats and hold straw at tip of open end. Submerge in 37-38 degree C. water bath. Do not let water get into the open end of the straw.
5.
Remove straw when air bubbles move freely when you invert straw. This usually takes 10-15 seconds.
6.
Dry straw completely on paper towel immediately.
7.
Do subsequent straws using same method.
8.
Hold straw open end over the warmed 37-38 degree C. Thaw Media in the glass culture tube. Cut straw just below cotton plug. Semen will run into the Thaw Media.
9.
Let combined thawed semen and thaw media stand in the glass culture tube for 2 minutes in the 37-38 degree C water bath.
10.
Draw up with large bore needle or insemination rod. Immediately inseminate into bitch.
PLEASE do not hesitate to call with ANY questions! Carol Bardwick | 760.591.9909
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