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PREPARATION
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A.
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Prepare
37-38 degree Centigrade water baths. Two large styrofoam cups with four
inches of water work well.
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B.
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Warm
insemination rod, syringe, and needle to 37-38 degrees Centigrade in an
incubator. DO NOT SUBMERGE IN WATER
BATH!
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THAW
PROCEDURES
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1.
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Open
shipping tank. Locate aluminum cane labeled "Thaw media". Remove
small vial containing thaw media and warm to room temperature. Estimated
time: 10-15 minutes.
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2.
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Put
1/2 cc. of room temperature THAW MEDIA in each culture tube. (One culture
tube with 1/2 cc thaw media for each straw.) Place tubes in 37-38 degree
centigrade water bath. Keep these tubes at 37-38 degrees centigrade THIS
IS IMPORTANT!
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3.
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Have
37-38 degree centigrade water bath ready for thawing semen.
WARNING! REMOVE STRAW ONLY WHEN YOU ARE READY TO THAW! Each semen straw must be thawed at 37-38 degrees C. Monitor temperature carefully for each straw. |
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4.
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Use
tweezers or hemostats and hold straw at tip of open end. Submerge in 37-38
degree C. water bath. Do not let water get into the open end of the straw.
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5.
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Remove
straw when air bubbles move freely when you invert straw. This usually
takes 10-15 seconds.
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6.
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Dry
straw completely on paper towel immediately.
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7.
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Do
subsequent straws using same method.
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8.
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Hold
straw open end over the warmed 37-38 degree C. Thaw Media in the glass
culture tube. Cut straw just below cotton plug. Semen will run into the
Thaw Media.
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9.
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Let
combined thawed semen and thaw media stand in the glass culture tube for
2 minutes in the 37-38 degree C water bath.
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10.
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Draw up with large bore needle or insemination rod. Immediately inseminate into bitch. |
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PLEASE
do not hesitate to call with ANY questions! Carol Bardwick | 760.591.9909
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